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a database for proteins with an experimentally verified function
Björn M. Ursing and Frank H.J. van Enckevort

NOTE: EXProt Release 2.01 is the most recent non-redundant version.


EXProt (database for EXPerimentally verified Protein functions) is a new non-redundant database containing protein sequences for which the function has been experimentally verified. EXProt, Release 2.01 is a selection of 6491 entries which are described to have an experimentally verified function, from Pseudomonas Community Annotation Project (1; PseudoCAP), from E. coli genome and proteome database (2; GenProtEC) and from division Prokaryotes of the EMBL Nucleotide Sequence Database (3), Release 69.
The entries in EXProt all have a unique ID number and provide information about organism, protein sequence, functional annotation, link to entry in original database, and if known, gene name and link to references in PubMed.

The EXProt database can be searched with BLAST or FASTA with amino acid or nucleotide sequence as query sequence. Note that only the sequence goes into the field. EXProt database is also searchable in SRS6 at CMBI.

In a near future entries from the genome project of Lactobacillus plantarum by Wageningen Centre for Food Sciences (WCFS) will be added to EXProt.

EXProt is published in In Silico Biol. 2, 0001.
EXProt is most recently published in Nucleic Acids Research, 2002, Vol. 30, No. 1, p. 50-51.
Reprint (PDF) Version of this Article This publication can be used as reference to EXProt database.
The EXProt data file can be downloaded from our ftp-site (ftp://ftp.cmbi.umcn.nl/pub/EXProt/).


  1. Stover,C.K., Pham,X.Q., Erwin,A.L., Mizoguchi,S.D., Warrener,P., Hickey M.J., Brinkman,F.S.L., Hufnagle,W.O., Kowalik, D.J , Lagrou,M., Garber,R.L., Goltry,L., Tolentino,E., Westbrock-Wadman,S., Yuan,Y., Brody,L.L., Coulter,S.N., Folger,K.R., Kas,A., Larbig,K., Lim,R., Smith,K., Spencer,D., Wong,G.K.-S., Wu,Z., Paulsen,I.T., Reizer,J., Saier,M.H., Hancock,R.E.W., Lory,S. and Olson,M.V. (2000). Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature, 406, 959-964.
  2. Riley,M. (1998). Genes and proteins of Escherichia coli K-12. Nucleic. Acids. Res. 26, 54.
  3. Stoesser, G., Baker, W., Broek, A. van den, Camon, E., Garcia-Pastor, M., Kanz, C., Kulikova, T., Lombard, V., Lopez, R., Parkinson, H., Redaschi, N., Sterk, P., Stoehr, P., Tuli, M. A. (2001). The EMBL nucleotide sequence database. Nucleic Acids Res., 29, 17-21.

Björn M. Ursing, Frank H.J. van Enckevort, 8 Jan 2002