We are proud to host the first Complexome Profiling workshop, bringing together its leading experts.

Goal

The Goal of this workshop is to assess the state of the art in complexome profiling, and to discuss future developments of the method. We aim to develop standards for the storage and annotation of complexomics data, to promote and facilitate sharing and reuse.

To achieve this, we will bring together the leading experts currently using this method. The state of the art in complexome profiling will be discussed, and together we will reach a consensus on the Minimum Information About a Complexomics Experiment (MIACE).

Programme

Friday 22 November 2019

13:00 - 14:30 Arrival and welcome
14:30 - 18:15

Session: Protein complex assembly and dynamics

session chair: Prof. dr. Martijn Huynen

speakers
  • 14:30Prof. Dr. Volker Zickermann
    • Assembly of respiratory complex I -- Interaction of three accessory subunits with assembly factor NDUFAF2
  • 15:10Dr. Cristina Ugalde
    • Multiple pathways coordinate the assembly of human mitochondrial complex IV and respiratory supercomplexes
  • 15:50Dr. Ilka Wittig
    • The dynamic complexome: Turnover within protein complexes in health and disease
  • 16:30Short break
  • 16:50Prof. Dr. Marcus Krüger
    • Complexome profiling of heart mitochondria with short capillary LC-MS gradients
  • 17:30Dr. Stefanie Berger
    • Complexomics of methane oxidizing archaea
19:00 - 20:00 Dinner
20:00 - Drinks at hotel bar

Saturday 23 November 2019

7:30 - 9:00 Breakfast
9:00 - 12:30

Session: Complexome profiling of non-mammalian organisms

session chair: Dr. Susanne Arnold

speakers
  •   9:00 Prof. Dr. Hans-Peter Braun
    • Complexome profiling for investigating mitochondrial protein complexes in plants
  •   9:40 Dr Etienne Meyer
    • Assembly of the OXPHOS complexes in plants
  • 10:20 Dr. Lars Wöhlbrand
    • Profiling membrane-protein complexes of marine, sulfate-reducing bacteria
  • 11:00 Dr. Mercedes Pardo Calvo
    • Landscape of the Plasmodium interactome
  • 11:40 Felix Evers Msc
    • Complexome profiling reveals Plasmodium falciparum mitochondrial protein dynamics
12:30 - 13:30 Lunch
13:30 - 15:30

MIACE workshop

Reaching a consensus on standards for annotation and reporting of complexomics data

15:30 - 16:00

Short Break

16:00 - 18:45

Highlight: Computational tools in complexomics

Showcasing computational tools for the analysis of complexome profiling data

session chair: Prof. dr. Ulrich Brandt

speakers
  • 16:00 Dr. Johannes Jordan
    • Interactive Exploration of Multi-dimensional Omics Data: The "Belki" Visualization Framework
  • 16:40 Dr. Uwe Schulte
    • Precise label-free quantification of LC-MS/MS shotgun proteomics data and its application to csBNMS
  • 17:20 Petra Palenikova
    • R package for analysis of SILAC complexomics data
  • 18:00 Joeri van Strien
    • Complexome Profiling Alignment
19:00 - 20:00 Dinner
20:00 - Drinks at hotel bar

Sunday 24 November 2019

7:30 - 9:00 Breakfast
9:00 - 12:30

Session: Innovative complexome profiling approaches

session chair: Joeri van Strien

speakers
  •   9:00 Dr. Sergio Guerrero-Castillo
    • Tandem mass tags for multiplexing complexome profiling
  •   9:40 Dr. Erika Fernandez-Vizarra
    • SILAC combined with complexome profiling to study OXPHOS assembly defects
  • 10:20 Dr. Hendrik Nolte
    • SILAC based temporal complexome profiling
  • 11:00 Dr. Uwe Schulte
    • High-resolution complexome profiling by cryo-slicing blue native gel electrophoresis coupled to mass spectrometry (csBN-MS)
  • 11:40 Dr. Rohan A. Thakur
    • Novel diaPASEF methods for improving data completeness in proteomics
12:30 - 13:30 Closing lunch
13:30 Departure

Confirmed Attendees

We are happy to announce the presence of the following workshop attendees:
  • Prof. Dr. Hans-Peter Braun
  • Institute of Plant Genetics, Leibniz University Hannover
    • Topic:
    • Complexome profiling for investigating mitochondrial protein complexes in plants
  • Prof. Dr. Marcus Krüger
  • Institute for Genetics, University of Cologne
    • Topic:
    • Complexome profiling of heart mitochondria with short capillary LC-MS gradients
  • Dr. Uwe Schulte
  • Institute of Physiology, University of Freiburg
    • Topic:
    • High-resolution complexome profiling by cryo-slicing blue native gel electrophoresis coupled to mass spectrometry (csBN-MS)
  • Dr. Johannes Jordan
  • Institute of Physiology, University of Freiburg
    • Topic:
    • Interactive Exploration of Multi-dimensional Omics Data: The "Belki" Visualization Framework
  • Prof. Dr. Berndt Fakler
  • Institute of Physiology, University of Freiburg
  • Dr Etienne Meyer
  • Institute for Plant Physiology, Martin-Luther-University, Halle-Wittenberg
    • Topic:
    • Assembly of the OXPHOS complexes in plants
  • Dr. Erika Fernandez-Vizarra
  • MRC-Mitochondrial Biology Unit, University of Cambridge
    • Topic:
    • SILAC combined with complexome profiling to study OXPHOS assembly defects
  • Dr. Sergio Guerrero-Castillo
  • Center for Obstetrics, Paediatrics and Youth Medicine, University Medical Center Hamburg-Eppendorf
    • Topic:
    • Tandem mass tags for multiplexing complexome profiling
  • Dr. Taco Kooij
  • Department of Medical Microbiology, Radboud University Medical Center
  • Felix Evers Msc
  • Department of Medical Microbiology, Radboud University Medical Center
    • Topic:
    • Complexome profiling reveals Plasmodium falciparum mitochondrial protein dynamics
  • Dr. Alfredo Cabrera-Orefice
  • Department of Pediatrics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
  • Dr. Hendrik Nolte
  • Max Planck Institute for Biology of Ageing, Cologne
    • Topic:
    • SILAC based temporal complexome profiling
  • Dr. Ilka Wittig
  • Functional Proteomics Core Unit, Goethe University
    • Topic:
    • The dynamic complexome: Turnover within protein complexes in health and disease
  • Dr. Lars Wöhlbrand
  • Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg
    • Topic:
    • Profiling membrane-protein complexes of marine, sulfate-reducing bacteria
  • Dr. Cristina Ugalde
  • University Hospital 12 de Octubre Research Institute, Madrid
    • Topic:
    • Multiple pathways coordinate the assembly of human mitochondrial complex IV and respiratory supercomplexes
  • Dr. Mercedes Pardo Calvo
  • Functional Proteomics, Institute of Cancer Research, London
    • Topic:
    • Landscape of the Plasmodium interactome
  • Andrew Sung
  • University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA
  • Dr. Rachel Guerra
  • Morgridge Institute for Research, Madison, Wisconsin, USA
  • Kristýna Čunátová
  • Department of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
  • Dr. Hans Spelbrink
  • Radboud Center for Mitochondrial Medicine, Department of Pediatrics, Radboud University Medical Center
  • Dr. Stefanie Berger
  • Department of Microbiology, Institute for Water and Wetland Research, Radboud University
    • Topic:
    • Complexomics of methane oxidizing archaea
  • Petra Palenikova
  • MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK
    • Topic:
    • R package for analysis of SILAC complexomics data
  • Prof. Dr. Volker Zickermann
  • Structural Bioenergetics Group, Institute of Biochemistry II, Goethe University Medical School
    • Topic:
    • Assembly of respiratory complex I -- Interaction of three accessory subunits with assembly factor NDUFAF2
  • Dr. Rohan A. Thakur
  • Executive Vice President, Life Sciences Mass spectrometry, Bruker Daltonik
    • Topic:
    • Novel diaPASEF methods for improving data completeness in proteomics
The organisers of this workshop:
  • Prof. dr. Martijn Huynen
  • Center for Medical and Biomedical Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
  • Prof. dr. Ulrich Brandt
  • Department of Pediatrics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
  • Dr. Susanne Arnold
  • Department of Pediatrics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
  • Joeri van Strien Msc
  • Center for Medical and Biomedical Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

Information about the Venue

Parkhotel Val Monte

Near the bustling city of Nijmegen and close to the German border in the village of Berg en Dal, you will find the Fletcher Parkhotel Val Monte. We are surrounded by hills, forests and have a magnificent view over the Ooijpolders nature reserve in Gelderland.

Location and contact:

Oude Holleweg 5
6572 AA Berg en Dal
+31 (0)24 - 684 20 00
info@parkhotelvalmonte.nl

Travel Information

Parkhotel Val Monte is easily accessible by public transport bus from Nijmegen Central Station. Plan your trip here.
For more information, visit their website.

Contact

For any questions or comments, please contact the organisation through Email

Funding

This project was made possible by: